?Genotype differences towards lead chloride harmful action

The aim of the study was to throw more light on PbCl 2 mode action (MoA) depending genotype by application three model organisms and microbiological, biochemical, molecular approaches. Three systems – Chlamydomonas reinhardtii strain 137C wild type (WT), Saccharomyces cerevisiae D7ts1, Pisum sativum L. cultivar Ran1 two experimental schemes short- long-term treatments were used. C. S. cell suspensions (1×10 6 cells/ml) at end exponential beginning a stationary phase growth treated with various concentrations (0.45–3.6 mM) for hours. Lower (0.03–0.22 also tested 137C. Short-term treatment up days in range 0.45–3.6 mM 10 0.45–2.7 performed P. seeds plants, respectively. Long-term concentration 3.6 not because very strong toxic effect (plant death). following endpoints used : survival, “visible” mutations, DNA double-strand breaks (DSBs), malondialdehyde (MDA), intracellular peroxides (H O ), photosynthetic pigments; gene conversion, reverse mutation, mitotic crossing-over, DSBs, superoxide anions, MDA glutathione (GSH); germination root length (short-term treatment), pro-oxidative markers MDA, H pigments (long-term treatment). Genotype differences between (0.047 (1.66 observed endpoints: inducing 50% lethality (LD 50 ) DSB induction. By contrast, no mutagenic found both unicellular test models. A slight capacity , measured as inhibition seed around 20% reduction revealed after equal or higher than 1.8 mM. variety stress responses plant models demonstrated comparing . dose-dependent increase levels minor (around 9–15%) when cells LD 20 –LD 80 (0.03–0.11 mM). Analyzing kinetics pea leaves, most pronounced shown 2.7 decrease detected designs short-term treatments. potential proven based increased anions decreased GSH. New information is gained that can affect molecule via induction oxidative stress. Our magnitude response towards genotype-specific. finding sensitive system contributes good strategies revealing low contaminants present chronically main environmental matrices. This first report, far we know, affirming induce DSBs